Authors: Tahira Mughal1, Ismat Naeem2, Sadia Qureshi 3, Ammara Abass2

Keywords: Heliotropium sterigosum is an annual herb found in south Punjab of Pakistan. Extract ofwhole plant in methanol was tested for anti bacterial activity against Staphylococcusaureus, Escherichia coli, Pseudomonas aeruginosa, Streptococcus pneumoniae, Bacillussubtilus, and Sarcina lutae. Minimum Inhibitory Concentration of methanolic extract wasdetermined and found to be bactericidal in concentration of 1000 µg/ml againststreptococcus pneumoniae, Escherichia coli, and Streptococcus pneumoniae. Synergisticantibacterial activity of methanolic extracts was tested with respective solvent extracts ofseeds of Withania coagulans, whole plant of Hypericum perforatum and Pinus roxburgii(bark). Staphylococcus aureus was synergistically inhibited by methanolic extract ofHeliotropium sterigosum and Pinus roxburgii (bark) (1µg/ml) and Withania coagulans(1µg/ml). Methanolic extract of Heliotropium sterigosum inhibits the growth of Sarcinalutae in combination with Pinus roxburgii (bark) methanolic extract (0.5µg/ml). Highestsynergistic activity was observed by Heliotropium sterigosum and Withania coagulansmethanolic extracts against Pseudomonas aeruginosa (0.5µg/ml) and Bacillus subtilus(1µg/ml).